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dos.5. Ca 2+ imaging for the neonatal OHCs

by Kurt Johnson

dos.5. Ca 2+ imaging for the neonatal OHCs The brand new rodent Ocm gene (series into the Shape S01) are cloned into pcDNA3.1CFP and you can pEGFP-N1 Flag (Addgene #60360). Next, EGFP-Flag try eliminated playing with BamHI (NEB) and you may NotI (NEB) and replaced with this new mCherry sequence (gene take off out of […]